./makeblastdb -in /Volumes/Bay3/Software/ncbi-blast-2.2.26+/db/uniprot_sprot -dbtype prot -out /Volumes/Bay3/Software/ncbi-blast-2.2.26+/db/uniprot_sprot
./blastx -query /Volumes/Bay3/Software/ncbi-blast-2.2.26+/query/QPXconsensus_21280.fa -db /Volumes/Bay3/Software/ncbi-blast-2.2.26+/db/uniprot_sprot -outfmt 6 -out /Volumes/Bay3/Software/ncbi-blast-2.2.26+/out/QPXconsensus_21280_SP_blastnout -num_threads 4
##### should have evalued that
-evalue 0.00005
--
galaxy ready
into Galaxy
Want to go back to node two an increase hits to 10.
Running on Node 2
Data @
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Lets also run nt
./blastn -num_threads 16 -out /Users/admin/Dropbox/Cluster/BLAST/output/QPX_nt_node2_blastout_hit10 -db /CLC_blastdatabases/nt -query /Users/admin/Dropbox/Cluster/BLAST/query/QPXconsensus_21280.fa -outfmt 6 -max_target_seqs 10 -evalue 0.00005 -task blastn